Isolation of carbon- and nitrogen-deprivation-induced loci of Sinorhizobium meliloti 1021 by Tn5-luxAB mutagenesis
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چکیده
منابع مشابه
Isolation of carbon- and nitrogen-deprivation-induced loci of Sinorhizobium meliloti 1021 by Tn5-luxAB mutagenesis.
Soil bacteria, such as Sinorhizobium meliloti, are subject to variation in environmental conditions, including carbon- and nitrogen-deprivation. The ability of bacteria to sense changes in their environment and respond accordingly is of vital importance to their survival and persistence in the soil and rhizosphere. A derivative of Tn5 which creates transcriptional fusions to the promoterless lu...
متن کاملFormation of chloroform and tetrachloroethene by Sinorhizobium meliloti strain 1021.
UNLABELLED The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chloroform (CHCl3 ) and 807·8 ± 13·5 ng l(-1) headspace volume of tetrachloroethene ...
متن کاملIsolation and symbiotic characterization of transposon Tn5-induced arginine auxotrophs of Sinorhizobium meliloti.
Seventeen arginine auxotrophic mutants of Sinorhizobium meliloti Rmd201 were isolated by random transposon Tn5 mutagenesis using Tn5 delivery vector pGS9. Based on intermediate feeding studies, these mutants were designated as argA/argB/argC/argD/argE (ornithine auxotrophs), argF/argI, argG and argH mutants. The ornithine auxotrophs induced ineffective nodules whereas all other arginine auxotro...
متن کاملProteomic analysis of rice seedlings infected by Sinorhizobium meliloti 1021.
Rhizobial endophytes infect and colonize not only leguminous plants, but several non-leguminous species as well. Using green fluorescent protein tagging technique, it has been shown that Rhizobia infect different varieties of rice species and migrate from plant roots to aerial tissues such as leaf sheaths and leaves. The interaction between them was found to promote the growth of rice. The grow...
متن کاملSmc01944, a secreted peroxidase induced by oxidative stresses in Sinorhizobium meliloti 1021.
Sequencing of the Sinorhizobium meliloti strain 1021 genome led to the detection of 6204 open reading frames, 41 % of which have no hypothetical function. To help annotate this genome, a transcriptome analysis was carried out with a dedicated microarray consisting of 146 genes belonging to three different classes: (i) no hypothetical function; (ii) potentially involved in oxidative stress respo...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1998
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-144-11-3205